53rd Congress of the European Societies of Toxicology (EUROTOX 2017), September 10 – 13, 2017, Bratislava, Slovak National Theatre
Sebastian Eggert, Joachim Wiest: Extension of the cytosensor microphysiometer test method toward cell impedance measurement
The cytosensor microphysiometer test method was validated by the European Commission for Validation of Alternative Methods to identify severe and not classified eye irritating chemicals. The corresponding INVITTOX # 130 protocol was adapted to the IMOLA-IVD technology. This method avoids the use of fetal bovine serum and is fully automated. Recent developments expanded the method toward a wider dilution series and the additional measurement of changes in cellular morphology using impedance measurement with inter-digitated impedance structures (IDES). The combination of extracellular acidification measurement as a metabolic parameter and impedance measurement as a morphological parameter yields better prediction capability to determine the eye irritation potential of new chemicals. First multi-parametric measurements show comparable MRD50 (metabolic rate decrement by 50%) values of sodium dodecyl sulfate (SDS). However, an effect of SDS toward the cellular impedance can only be detected at a higher concentration. The expanded protocol will be used to create a new prediction model and it will be evaluated if it is possible to address the whole range of classification in the field of eye irritation.