Skin-on-a-Chip at ESTIV 2018

Please visit our presentation “Skin-on-a-chip with an automated air-liquid interface” at the 20th International Congress on In Vitro Toxicology (ESTIV 2018) in Berlin.

 

Abstract

We present a label-free solution that leverages the use of the intelligent mobile lab for in vitro diagnostics (IMOLA-IVD), a noninvasive, sensor-based platform, to monitor the transepithelial electrical resistance (TEER) of RhE models and adherent cells cultured on porous membrane inserts. Murine fibroblasts cultured on polycarbonate membranes were first used as a test model to optimize procedures using a custom BioChip encapsulation design, as well as dual fluidic configurations, for continuous and automated perfusion of membrane-bound cultures. Extracellular acidification rate (EAR) and TEER of membrane-bound L929 cells were monitored. The developed protocol was then used to monitor the TEER of MatTek EpiDermTM RhE models over a period of 48 h [1].

1 Alexander, F., Eggert, S., Wiest, J.: Skin-on-a-chip: Transepithelial electrical resistance and extracellular acidification measurements through an automated air-liquid interface, Genes, 2018, 9/2, 114; doi:10.3390/genes9020114

Lush Prize 2018

cellasys is delighted to be shortlisted for the Lush Prize 2018 in the category science:

shorlist Lush Prize 2018

Analysis software released

The DALiA analytics 1.0 software is available.

DALiA analytics processes data from our microphysiometric systems.

Please see a short demo here:

IEEE EMBC 2018

Innovative through research

The Stifterverband für die Deutsche Wissenschaft awards the cachet “INNOVATIVE THROUGH RESEARCH” to cellasys.

Microphysiometry

Microphysiometry

Time-resolved monitoring of metabolic activities in vitro (microphysiometry) is necessary to study the dynamic regulations of core biochemical pathways in cellular disease models. Following a brief review of recent developments in the field, this contribution presents the most important branches of current sensor-based methods of microphysiometry. The primary parameters assessed by microphysiometry are extracellular pH changes and the concentration of dissolved oxygen to conclude on extracellular acidification (EAR) and oxygen uptake rates (OUR) as quantitative measures for cell metabolism. Direct sensing of selected small molecule metabolites or metabolic heat are alternative assay strategies. The major physical transduction principles encompass electrochemical and optochemical sensing complemented by less popular approaches for highly specific applications. All microphysiometric devices include tissue culture maintenance systems that have to guarantee physiological conditions and that must be functionally aligned with the sensing component. The interplay of cell metabolic activity and sensors in microscaled reaction volumes can be simulated with appropriate numerical models describing the physical processes of reaction and diffusion. While aspects of automation and throughput belong more to the engineering side of microphysiometry, both form the basis for the inevitable statistical data acquisition and analysis. This paper concludes with a description of two selected applications of microphysiometry, one in toxicology and the other one in clinical cancer research.

https://doi.org/10.1007/11663_2018_2

analytica 2018

cellasys will not have a booth at this years analytica in Munich …

However, you are welcome to visit our microphysiometric systems in our facilities in Munich – Schwabing during analytica 2018.

Please arrange your visit via info@cellasys.com.

 

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LOPEC 2018

You are welcome to visit our presentation at LOPEC 2018:

Low-cost biosensors with printed interdigitated graphene electrodes

Thomas Velten1, Thorsten Knoll1, Axel Brenner1 Anke Schultz1, Joachim Wiest2, Renate Warmers3, Gerald Jenke3, Anna Zumbülte3, Andreas Urban4, Kris Seunarine5

1Fraunhofer-Institute for Biomedical Engineering, 2cellasys GmbH, 3Saueressig GmbH + Co. KG, 4AiCuris Anti-infective Cures GmbH, 5Haydale Limited

Cell-based assays are of enormous importance in anti-viral drug discovery and research on vaccines against viral infections. Therefore, the inhibition of the virus-induced cytopathic effect due to neutralizing antibodies or antiviral agents comprise to the commonly utilized methods. The impedance spectroscopy with commercial platforms demonstrated the principal applicability of this technique for the mentioned applications in well plate format. But costs of disposable components of these systems are high due to the used fabrication technology. We aim at printing low-cost sensor systems for applications in antiviral drug testing and toxicity testing.