The 1st Conference on Science and Engineering for Instrumentation, Environment and Renewable Energy 2015 themed “Science and Engineering for Enhancing Quality of Life and Ensuring a Better Future” will be held in Pekanbaru, Indonesia at the University of Riau, September 28-29, 2015. It is jointly organized by the University of Riau (UR) Indonesia, cellasys GmbH and the Technische Universitaet Muenchen (TUM) Germany.
Welcome to our presentation at the “Lab-on-a-Chip, Microfluidics & Microarrays World Congress” in San Diego / USA.
In the presented work, a candidate 3D in vitro culture, the reconstructed human epidermis (RhE) artificial skin, was integrated with a modified IMOLA-IVD biochip for online monitoring of extracellular acidification. RhE, grown on standard polycarbonate membrane culture inserts, mimic the structure and function of human epidermis. Monitoring the metabolic output of an RhE in real-time can reveal time-resolved data on the toxic effect new compounds have on the epidermis. Automatic screening can also provide a new pathway that can revolutionize the process of testing new chemicals for skin irritancy. For this reason, a protocol for an automated skin corrosion/irritation assay was developed. IMOLA Biochip-D’s were modified to perfuse cell culture inserts with medium while maintaining an air-liquid interface. Metabolic signals were recorded in real-time in an incubator via the IMOLA-IVD system. RhE from CellSystems GmbH (epiCS) on culture inserts were perfused automatically with medium via a peristaltic pump, IMOLA fluidic modules and the DALiA control software. Future work will involve developing this method into a validated irritancy and corrosivity assay following from OECD testing guideline 431 (corrosion) and 439 (irritation), by exposing the epiCS to potentially toxic agents.
Meet us at the EUSAAT 2015 in Linz / Austria.
cellasys has four contributions to the congress:
28-day microphysiological monitoring of human hepatocellular cells
Analysis of effects of the adoption of alternative methods on the number of animals used for eye irritation in the European Union
Reconstructed Human Epidermis (RhE) Monitoring via the IMOLA-IVD
Conversion of the draft OECD guideline for the Testing of Chemicals: The Cytosensor Microphysiometer Test Method
You are welcome to visit our invited session on “Label Free Live Cell Monitoring” during the 37th Annual International Conference of the IEEE Engineering in Medicine and Biology Society in Milan.
IEEE EMBC 2015 Session “Label Free Live Cell Monitoring”:
Martin Brischwein, Technische Universität München:
Sensor Based Microphysiometry
The capability of continuously monitoring cells and tissues in real-time for hours or days supports the value of a sensor-based microphysiometric approach. While there are widespread applications now for in-vitro settings, the use for smart, implanted devices is just beginning. The spectrum of analysed functional parameters comprises cellular morphological dynamics, metabolic activity and patterns of electric activity. An outline of a study on human tumor tissue samples gives an example of the potential benefits and challenges of in-vitro microphysiometry.
Joachim Wegener, Universität Regensburg:
Impedance Analysis of Different Cell Monolayers Grown on Gold-Film Electrodes
Impedance analysis of mammalian cells grown on planar film electrodes provides a label-free, non-invasive and unbiased observation of cell-based assays addressing the biological response to drugs, toxins or stressors in general. Whereas the time course of the measured impedance at one particular frequency has been used a lot for quantitative monitoring, in-depth analysis of the frequency-dependent impedance spectra is rarely performed. This study summarizes and validates the existing model for spectral analysis by applying it to eight different cell types from different mammalian tissues. Model parameters correctly predict the functional and/or structural properties of the individual cells under study.
Frank Alexander, cellasys GmbH:
Online, Label-Free Monitoring of Organ-On-A-Chip Models: The Case for Microphysiometry
Primarily composed of cells on a porous membrane embedded in microfluidic channels, organ-on-a-Chip (OOC) models are coming into the spotlight as an innovative, new approach to in vitro modeling. However, more work is required to understand the impact OOCs have on cellular function including basal metabolism, barrier resistance and oxygen consumption. Electrochemical sensor-based cellular microphysiometry provides a noninvasive, real-time methodology for monitoring these attribute and can be applied to develop robust, automated assays for organ toxicology. To date, few OOCs have been studied with integrated electrochemical sensors. In this presentation, we define organ-on-a-chip systems, outline which have been studied with integrated sensors, and present a novel method to study cells cultured directly on a porous membrane.
Cornelia Pfister, HP Medizintechnik GmbH:
Dynamic Monitoring of Cellular Metabolic Activity in Combination with Live Cell Imaging
We present an automated analysis of the cellular dynamic metabolic activity in combination with live cell imaging, an essential factor for understanding the fundamental cellular physiological responses. Therefore, we utilized the Intelligent Microplate Reader, a new analysis platform for marker-free cell-based assays in real-time. To demonstrate the benefit of the platform, we analyzed the relationship between various dynamic cell parameters (extracellular acidification, oxygen uptake, cell morphology, cell density and cell migration) of L929, a mouse fibroblast cell line, under the influence of sodium dodecyl sulfate. The dynamic kinetics of the monitored parameters are consistent and revealing much information about the transactions occurring in the cells.
Lazuardi Umar, University of Riau:
Application of Algae-Biosensor for Environmental Monitoring
Environmental problems including water and air pollution, over fertilization, insufficient wastewater treatment and even ecological disaster are receiving greater attention in the technical and scientific area. In this paper, a method for water quality monitoring using living green algae (Chlorella Kessleri) with the help of the intelligent mobile lab (IMOLA) is presented. This measurement used two IMOLA systems for measurement and reference simultaneously to verify changes due to pollution inside the measurement system. The IMOLA includes light emitting diodes to stimulate photosynthesis of the living algae immobilized on a biochip containing a dissolved oxygen microsensor. A fluid system is used to transport algae culture medium in a stop and go mode; 600s ON, 300s OFF, while the oxygen concentration of the water probe is measured. When the pump stops, the increase in dissolved oxygen concentration due to photosynthesis is detected. In case of a pollutant being transported toward the algae, this can be detected by monitoring the photosynthetic activity. Monitoring pollution is shown by adding emulsion of 0,5mL of Indonesian crude palm oil and 10mL algae medium to the water probe in the biosensor.
Johannes Clauss, Technische Universität München:
In-Vivo Cell and Tissue Monitoring with Active Implants
Active implant systems are becoming increasingly important in modern medicine. We describe the development of an implantable system for the monitoring of dissolved oxygen. Tissue oxygen saturation plays a leading role in many pathophysiological processes in the human body such as the growth of malignant tumors or the viability of transplanted organs. The implant allows monitoring the tissue oxygenation in vivo with a wireless interface to an external device. An improved self-calibration technique is described to minimize sensor drift with electrochemical sensors in vivo for a better long term stability of the implant system. The sensor was coated with a hydrogel membrane to avoid convection artifacts during calibration procedure.
cellasys at ACHEMA 2015 in Frankfurt / Germany.
Vist us at the both of HP Medizintechnik GmbH (Hall 4.1 – D49) in Frankfurt / Germany from 15th to 19th of June 2015.
INDUSTRIAL WORKSHOP: Higher Value production technologies and KET enabled applications
Date: 30. March 2015
Venue: Assolombarda Sala Falck, Via Chiaravalle 8, Milano, Italy
Within the European initiative “Factories of the Future”,a group of public private partnership projects is working in synergy to maintain high value manufacturing in Europe. These projects together with the support of the European Commission have created a “Micro -Manufacturing Industrial Research” Cluster. The Cluster brings together a critical mass of industry facing R&D programmes with common development objectives and complementary technologies to underpin a number of key application areas for Europe competitiveness.
cellasys will present two posters at the “9th World Congress on Alternatives and Animal Use in the Life Sciences“:
1. Serial Linkage of BioChips toward an organ-on-chip approach
2. Using chlorella kessleri for monitoring of water quality
Serial Linkage of BioChips toward an organ-on-chip approach
The development of drugs is a costly and time consuming process. Although high throughput methods based on cell cultures are available, they only give a hint on the real effect of a future drug. Traditional two dimensional cell cultures are not able to reproduce/emulate the complex interactions of different types of tissue like it exists in the human body. Such interactions include the absorption, distribution, metabolism and elimination (ADME) of a drug. Recently new approaches reforming the cell culture techniques were published. Most of them are based on several microchambers that are cultivated with different cells. Microfluidic channels are used to link these chambers and to ensure exchange of metabolites. With such devices e.g. primary cells can be cultivated for a longer time period and with a smaller loss of functionality than in former single-type 2D cell-cultures. A lable-free long term monitoring of organ-on-chip systems would affirm the benefits of such systems. Electrochemical sensors are well suited for this task, but need to be careful integrated. As proof-of-concept we serially linked two electrochemical cell-monitoring systems (IMOLA-IVD, cellasys GmbH) and showed that the exchange of metabolites can be monitored without any crosstalk in a label-free, long-term and multi-parametric manner.
Using chlorella kessleri for monitoring of water quality
Polution of surface waters is an increasing problem in the modern world. Causes are various like over-fertilization, insufficient capabilities or lack of wastewater treatment plants, littering, ecological disaster or eaven chemical and biological warfare. In the presented work the algae chlorella kessleri was used as an unspecific signal transducer for water quality. The algae was immoilized on a BioChip with a cellulose membrane, supplied with algae culture broth and monitored using the IMOLA-IVD technology which was developed in cooperation with the Heinz Nixdorf Lehrstuhl für Medizinische Elektronik at Technische Universität München. Here, the oxygen production due to photosynthetic stimulation of the algae with light emitting diodes and the extracellular acidification is monitored. If the water quality changes, the photosynthetic activity of the algae is also altered and that can be detected with the set-up. In a proof of principle study, probes from Indonesian palm plants were investigated. The technology showed that it can be used as an unspecific early warning system for water quality monitoring. The technology and first results using probes from Indonesian palm plants are presented. Future work will be on the transition from the laboratory to a real world scenario.
New premises for cellasys – R&D
cellasys – R&D moved to new premises at
cellasys – R&D
80802 Munich Germany
BioChip-D data sheet available.
The data sheet of our new BioChip-D is available. The BioChip is an improved version of the BioChip-C. The substrate is made of glass and allows access via optical techniques.
“All-in-one manufacturing platform for system in package and micromechatronic systems” – project website is availabe at www.nextfactory-project.eu
The NextFactory project (funded from the European Union’s Seventh Framework Programme) aims to develop one machine which is able to produce complete mechatronic micro-systems in an integrated, single-stage, one-location process.
More information in the NextFactory-Factsheet.
87758 Kronburg / Germany
cellasys GmbH – R&D
80802 Munich / Germany
Tel.: +49 89 200011074
Fax.: +49 89 200011076